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Image Search Results
Journal: Reproduction
Article Title: Stable inhibition of interleukin 1 receptor type II in Ishikawa cells augments secretion of matrix metalloproteinases: possible role in endometriosis pathophysiology
doi: 10.1530/rep-06-0377
Figure Lengend Snippet: Figure 2 Immunofluorescence analysis of IL1R1, IL1R2, and IL1RAcP expression in non-transfected Ishikawa cells (NT) and E03, E11, A08, and A17 clones. Note the com- parable intensity of IL1R1 and IL1RAcP immunofluorescent signals in NT, E03, and E11 clones, and the marked decline in IL1R2 immunofluorescent signal in A08 and A17 clones. No immunofluorescence was observedinnegativecontrols(c)in theabsence of primary antibodies (objective !40).
Article Snippet:
Techniques: Expressing, Transfection, Clone Assay
Journal: Reproduction
Article Title: Stable inhibition of interleukin 1 receptor type II in Ishikawa cells augments secretion of matrix metalloproteinases: possible role in endometriosis pathophysiology
doi: 10.1530/rep-06-0377
Figure Lengend Snippet: Figure 3 Representative Western blot analysis of IL1R1 (A), IL1R2 (B), and IL1RAcP (C) expression in non-transfected Ishikawa cells (NT) and E03, E11, A08, and A17 clones. Equal amounts of proteins were subjected to SDS-PAGE analysis and Western blotting. Recombinant IL1R1, IL1R2, and IL1RAcP proteins were taken as controls (rR). Blotting with anti-tubulin antibody demonstrates equal protein loading. Note the reduced intensity of IL1R2 bands corresponding to either the membrane-bound or the soluble forms of the receptor, when compared with NT cells, and the absence of noticeable change in IL1R1 and IL1RAcP expression; mb, membrane-bound; s, soluble.
Article Snippet:
Techniques: Western Blot, Expressing, Transfection, Clone Assay, SDS Page, Recombinant, Membrane